RESEARCH PAPER
Selected metalloproteinases and their inhibitors in prediction of nifedypine tocolysis effectiveness
1
Zakład Fizjopatologii, Instytut Medycyny Wsi, Lublin
2
Oddział Położniczy i Trakt Porodowy, Samodzielny Publiczny Szpital Wojewódzki im. Jana Bożego, Lublin
3
Samodzielna Pracownia Badań Izobolograficznych, Instytut Medycyny Wsi w Lublinie
4
Klinika Dermatologii, Wenerologii i Dermatologii Dziecięcej, Uniwersytet Medyczny, Lublin
Med Og Nauk Zdr. 2015;21(1):1-3
KEYWORDS
ABSTRACT
Introduction:
Matrix metalloproteinases and their inhibitors play an important role in pregnancy, childbirth and postpartum. This system allows inflammatory response cells migration to damaged tissues, release cytokines and cytokine receptors. Preterm cervical ripening is an inflammatory process, with cytokines as important mediators. Objective. The purpose of this study was to evaluate the relationship between metalloproteinases MMP -2 and MMP -9 and their inhibitors TIMP -1 and TIMP -2 in patients with preterm labour in the prediction of nifedypine tocolysis efficacy.
Material and Methods:
The study included 21 women with preterm labour and 35 healthy pregnant women below 37 weeks of gestation. The patients were divided into three groups: the first group consisted of patients with preterm labour with ineffective tocolysis, the second group of women with preterm labour responded to tocolysis, the third group consisted of healthy pregnant women. The level of serum MMP -2, MMP – 9 and TIMP1 and TIMP2 was assessed using the ELISA kit.
Results:
The concentration of MMP -2 in preterm labour patients was not significantly different compared to the control group. In women with a preterm labour level of MMP -9 was higher than in healthy pregnant women. The concentration of TIMP -1 and TIMP -2 in sera of pregnant women with preterm delivery was higher compared to the control group. It was found that in women with preterm labour with ineffective tocolysis, the level of TIMP -1 was higher, while the level of TIMP -2 was significantly lower compared to pregnant women with ineffective tocolysis.
Conclusions:
Ineffective tocolysis may be due to the stage of inflammation at the start of therapy. Activation of the proteolytic activity of gelatinase / inhibitors system may indicate an active process leading to cervix shortening and dilating in preterm labour, and increased activity of metalloproteinase inhibitors is probably a defence mechanism, as noted.
Matrix metalloproteinases and their inhibitors play an important role in pregnancy, childbirth and postpartum. This system allows inflammatory response cells migration to damaged tissues, release cytokines and cytokine receptors. Preterm cervical ripening is an inflammatory process, with cytokines as important mediators. Objective. The purpose of this study was to evaluate the relationship between metalloproteinases MMP -2 and MMP -9 and their inhibitors TIMP -1 and TIMP -2 in patients with preterm labour in the prediction of nifedypine tocolysis efficacy.
Material and Methods:
The study included 21 women with preterm labour and 35 healthy pregnant women below 37 weeks of gestation. The patients were divided into three groups: the first group consisted of patients with preterm labour with ineffective tocolysis, the second group of women with preterm labour responded to tocolysis, the third group consisted of healthy pregnant women. The level of serum MMP -2, MMP – 9 and TIMP1 and TIMP2 was assessed using the ELISA kit.
Results:
The concentration of MMP -2 in preterm labour patients was not significantly different compared to the control group. In women with a preterm labour level of MMP -9 was higher than in healthy pregnant women. The concentration of TIMP -1 and TIMP -2 in sera of pregnant women with preterm delivery was higher compared to the control group. It was found that in women with preterm labour with ineffective tocolysis, the level of TIMP -1 was higher, while the level of TIMP -2 was significantly lower compared to pregnant women with ineffective tocolysis.
Conclusions:
Ineffective tocolysis may be due to the stage of inflammation at the start of therapy. Activation of the proteolytic activity of gelatinase / inhibitors system may indicate an active process leading to cervix shortening and dilating in preterm labour, and increased activity of metalloproteinase inhibitors is probably a defence mechanism, as noted.
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| eISSN: | 2084-4905 |
| ISSN: | 2083-4543 |
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